Study of Antibiotic Resistance and Prevalence of bla-TEM gene in Klebsiella pneumoniae Strains isolated from Children with UTI in Tabriz Hospitals
Introduction: Klebsiella pneumoniae is one of the most important factors in children’s urinary tract infections. Resistance to routine antibiotics is a major problem in the therapeutic process. Therefore, this study was conducted to determine the antibiotic resistance and to investigate the prevalence of bla-TEM gene in K.pneumoniae isolated from urinary tract infections.
Methods: This cross-sectional descriptive study was performed on 120 samples of K.pneumonia isolated from children with urinary tract infection referring to hospitals in Tabriz. Samples were isolated and identified using standard laboratory methods and microbiology and culture in specific environments. Antibiotic resistance patterns of bacteria isolated by Kirby-Bauer disc diffusion method were performed. In order to identify the bla-TEM gene, the combined disk test was used as a phenotypic method and PCR method was used to identify the genotypes.
Results: The highest sensitivity to the antibiotics was Tazocin(88%) and Aztreonam (87%). The combined test results showed that 26 isolates producing Extended-Spectrum beta-lactamases, of which 22 were isolates with the bla-TEM beta-lactamase gene.
Conclusions: By increasing the prevalence of resistance to antibiotics, rapid and timely diagnosis of antibiotic-resistant strains is necessary in order to select appropriate treatments and to prevent the spread of resistance.