Green Synthesis and Characterization of Silver Nanoparticles Using Nyctanthes arbortristis Linn Leaf Extract and Their Anti Bacterial Activity


Abstract

Introduction: Green chemistry is the design of chemical products and processes that reduce the generation of hazardous substances. Since the last few years, natural products especially plant extracts have been extensively explored for their potency to synthesize silver nanoparticles (Ag NPs). The current study designed to synthesize Ag NPs adopting a green approach using Nyctanthes arbortristis Linn (N. arbortristis) aqueous leaf extract.
Methods: Synthesized Ag NPs were characterized by using UV-visible spectroscopy, scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR) and the prepared Ag NPs were also evaluated for the antibacterial activities.
Results: This study portrays a facile synthesis of N. arbortristis leaf extract and were noticed through visual color change to brown color. The achieved results authenticated with UV-Visible spectrum exhibited an absorption band at around 440 nm. The SEM images confirmed that the size of Ag NPs sample was 28-30 nm. FTIR results also confirmed the formation of Ag NPs. The Synthesized Ag NPs have enhanced antibacterial activities against both Bacillus cereus (gram positive) and gram negative bacteria (E.coli, Pseudomonas aeruginosa, Klebsiella sps).
Conclusions: The current study illustrated in a simple one step invention of Ag NPs with better control over the size of the material and the significant antibacterial activity was displayed.

Keywords

Green Synthesis; Silver Nanoparticles; Nyctanthes arbortristis Linn; Antibacterial Activity

Green Synthesis and Characterization of Silver Nanoparticles Using Nyctanthes arbortristis Linn Leaf Extract and Their Anti Bacterial Activity


Authors

Subramani Srinivasan

E-mail: [email protected]
Affiliation: Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar – 608 002, Tamilnadu, India; Postgraduate and Research Department of Biochemistry, Government Arts College For Women, Krishnagiri- 635 002, Tamilnadu, India

Kathiroli Sujithra

Affiliation: Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar – 608 002, Tamilnadu, India

Udaiyar Muruganathan

Affiliation: Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar – 608 002, Tamilnadu, India

Raju Murali

Affiliation: Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar – 608 002, Tamilnadu, India; Postgraduate and Research Department of Biochemistry, Government Arts College For Women, Krishnagiri- 635 002, Tamilnadu, India

Chandran Navetha

Affiliation: Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar – 608 002, Tamilnadu, India

Dhananjayan Indumathi

Affiliation: Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar – 608 002, Tamilnadu, India

Green Synthesis and Characterization of Silver Nanoparticles Using Nyctanthes arbortristis Linn Leaf Extract and Their Anti Bacterial Activity


References

1. Rathi Sre PR, Reka M, Poovazhagi R, Arul Kumar M, Murugesan K. Antibacterial and cytotoxic effect of biologically synthesized silver nanoparticles using aqueous root extract of Erythrina indica lam. Spectrochim Acta A Mol Biomol Spectrosc. 2015;135:1137-44. DOI: 10.1016/j.saa.2014.08.019 PMID: 25189525
2. Satterlee AB, Huang L. Current and Future Theranostic Applications of the Lipid-Calcium-Phosphate Nanoparticle Platform. Theranostics. 2016;6(7):918-29. DOI: 10.7150/thno.14689 PMID: 27217828
3. Chen D, Li X, Soule T, Yorio F, Orr L. Effects of solution chemistry on antimicrobial activities of silver nanoparticles against Gordonia sp. Sci Total Environ. 2016;566-567:360-7. DOI: 10.1016/j.scitotenv.2016.05.037 PMID: 27228306
4. He H, Cai R, Wang Y, Tao G, Guo P, Zuo H, et al. Preparation and characterization of silk sericin/PVA blend film with silver nanoparticles for potential antimicrobial application. Int J Biol Macromol. 2017;104(Pt A):457-64. DOI: 10.1016/j.ijbiomac.2017.06.009 PMID: 28619637
5. Xin L, Wang J, Zhang LW, Che B, Dong G, Fan G, et al. Development of HSPA1A promoter-driven luciferase reporter gene assays in human cells for assessing the oxidative damage induced by silver nanoparticles. Toxicol Appl Pharmacol. 2016;304:9-17. DOI: 10.1016/j.taap.2016.05.010 PMID: 27211842
6. Salunke GR, Ghosh S, Santosh Kumar RJ, Khade S, Vashisth P, Kale T, et al. Rapid efficient synthesis and characterization of silver, gold, and bimetallic nanoparticles from the medicinal plant Plumbago zeylanica and their application in biofilm control. Int J Nanomedicine. 2014;9:2635-53. DOI: 10.2147/IJN.S59834 PMID: 24920901
7. Pethakamsetty L, Kothapenta K, Nammi HR, Ruddaraju LK, Kollu P, Yoon SG, et al. Green synthesis, characterization and antimicrobial activity of silver nanoparticles using methanolic root extracts of Diospyros sylvatica. J Environ Sci (China). 2017;55:157-63. DOI: 10.1016/j.jes.2016.04.027 PMID: 28477809
8. Sasmal NK, Pahari TK, Laha R. Experimental infection of the cockroach Periplaneta americana with Toxocara canis and the establishment of patent infections in pups. J Helminthol. 2008;82(2):97-100. DOI: 10.1017/S0022149X07875936 PMID: 18053303
9. Saxena RS, Gupta B, Lata S. Tranquilizing, antihistaminic and purgative activity of Nyctanthes arbor tristis leaf extract. J Ethnopharmacol. 2002;81(3):321-5. DOI: 10.1016/s0378-8741(02)00088-0 PMID: 12127232
10. Das S, Sasmal D, Basu SP. Evaluation of CNS Depressant Activity of Different Plant parts of Nyctanthes arbortristis Linn. Indian J Pharm Sci. 2008;70(6):803-6. DOI: 10.4103/0250-474X.49129 PMID: 21369448
11. Sankar R, Karthik A, Prabu A, Karthik S, Shivashangari KS, Ravikumar V. Origanum vulgare mediated biosynthesis of silver nanoparticles for its antibacterial and anticancer activity. Colloids Surf B Biointerfaces. 2013;108:80-4. DOI: 10.1016/j.colsurfb.2013.02.033 PMID: 23537829
12. Mueller JH, Hinton J. A Protein-Free Medium for Primary Isolation of the Gonococcus and Meningococcus. Exp Biol Med. 1941;48(1):330-3. DOI: 10.3181/00379727-48-13311
13. Bhattacharya D, Gupta RK. Nanotechnology and potential of microorganisms. Crit Rev Biotechnol. 2005;25(4):199-204. DOI: 10.1080/07388550500361994 PMID: 16419617
14. Amarnath K, Kumar J, Reddy T, Mahesh V, Ayyappan SR, Nellore J. Synthesis and characterization of chitosan and grape polyphenols stabilized palladium nanoparticles and their antibacterial activity. Colloids Surf B Biointerfaces. 2012;92:254-61. DOI: 10.1016/j.colsurfb.2011.11.049 PMID: 22225943
15. Vijayakumar M, Priya K, Nancy FT, Noorlidah A, Ahmed ABA. Biosynthesis, characterisation and anti-bacterial effect of plant-mediated silver nanoparticles using Artemisia nilagirica. Ind Crops Prod. 2013;41:235-40. DOI: 10.1016/j.indcrop.2012.04.017
16. Raghunandan D, Mahesh BD, Basavaraja S, Balaji SD, Manjunath SY, Venkataraman A. Microwave-assisted rapid extracellular synthesis of stable bio-functionalized silver nanoparticles from guava (Psidium guajava) leaf extract. J Nanopart Res. 2010;13(5):2021-8. DOI: 10.1007/s11051-010-9956-8
17. Courrol LC, Silva FRDO, Gomes L. A simple method to synthesize silver nanoparticles by photo-reduction. Colloids Surfaces a-Physicochem Eng Aspects. 2007;305(1-3):54-7. DOI: 10.1016/j.colsurfa.2007.04.052
18. Njagi EC, Huang H, Stafford L, Genuino H, Galindo HM, Collins JB, et al. Biosynthesis of iron and silver nanoparticles at room temperature using aqueous sorghum bran extracts. Langmuir. 2011;27(1):264-71. DOI: 10.1021/la103190n PMID: 21133391
19. Basu S, Maji P, Ganguly J. Rapid green synthesis of silver nanoparticles by aqueous extract of seeds of Nyctanthes arbor-tristis. Appl Nanosci. 2015;6(1):1-5. DOI: 10.1007/s13204-015-0407-9
20. Najimu Nisha S, Aysha OS, Syed Nasar Rahaman J, Vinoth Kumar P, Valli S, Nirmala P, et al. Lemon peels mediated synthesis of silver nanoparticles and its antidermatophytic activity. Spectrochim Acta A Mol Biomol Spectrosc. 2014;124:194-8. DOI: 10.1016/j.saa.2013.12.019 PMID: 24486863
21. Ponce A, Mejia-Rosales S, Jose-Yacaman M. Scanning transmission electron microscopy methods for the analysis of nanoparticles.
Methods Mol Biol. 2012;906:453-71. DOI: 10.1007/978-1-61779-953-2_37 PMID: 22791456
22. Dutta PP, Bordoloi M, Gogoi K, Roy S, Narzary B, Bhattacharyya DR, et al. Antimalarial silver and gold nanoparticles: Green synthesis, characterization and in vitro study. Biomed Pharmacother. 2017;91:567-80. DOI: 10.1016/j.biopha.2017.04.032 PMID: 28486189
23. Paikara D, Singh S, Pandey B. Phytochemical Analysis of leave extract of Nyctanthes arbortristis. IOSR J Environ Sci Toxicol Food Technol 2015;1(3):39-42.
24. El-Sheikh MA. A novel photosynthesis of carboxymethyl starch-stabilized silver nanoparticles. ScientificWorldJournal. 2014;2014:514563. DOI: 10.1155/2014/514563 PMID: 24672325
25. Hu RL, Li SR, Kong FJ, Hou RJ, Guan XL, Guo F. Inhibition effect
of silver nanoparticles on herpes simplex virus 2. Genet Mol Res. 2014;13(3):7022-8. DOI: 10.4238/2014.March.19.2 PMID: 24682984
26. Gogoi N, Babu PJ, Mahanta C, Bora U. Green synthesis and characterization of silver nanoparticles using alcoholic flower extract of Nyctanthes arbortristis and in vitro investigation of their antibacterial and cytotoxic activities. Mater Sci Eng C Mater Biol Appl. 2015;46:463-9. DOI: 10.1016/j.msec.2014.10.069 PMID: 25492011

Green Synthesis and Characterization of Silver Nanoparticles Using Nyctanthes arbortristis Linn Leaf Extract and Their Anti Bacterial Activity


Citation tools

Vancouver

Srinivasan S, Sujithra K, Muruganathan U, Murali R, Navetha C, Indumathi D. Green Synthesis and Characterization of Silver Nanoparticles Using Nyctanthes arbortristis Linn Leaf Extract and Their Anti Bacterial Activity. Focus on Sciences. 2106; 3(4):1-6


APA

Srinivasan, S., Sujithra, K., Muruganathan, U., Murali, R., Navetha, C., & Indumathi, D. (2106). Green Synthesis and Characterization of Silver Nanoparticles Using Nyctanthes arbortristis Linn Leaf Extract and Their Anti Bacterial Activity. Focus on Sciences, 3(4), 1-6.


Chicago

Subramani Srinivasan, Kathiroli Sujithra, Udaiyar Muruganathan, Raju Murali, Chandran Navetha, and Dhananjayan Indumathi "Green Synthesis and Characterization of Silver Nanoparticles Using Nyctanthes arbortristis Linn Leaf Extract and Their Anti Bacterial Activity". Focus on Sciences 3, no. 4 (2106).

Green Synthesis and Characterization of Silver Nanoparticles Using Nyctanthes arbortristis Linn Leaf Extract and Their Anti Bacterial Activity


Share this article

To share this article click on each icone.