Study of Antibiotic Resistance and Prevalence of bla-TEM gene in Klebsiella pneumoniae Strains isolated from Children with UTI in Tabriz Hospitals


Abstract

Introduction: Klebsiella pneumoniae is one of the most important factors in children’s urinary tract infections. Resistance to routine antibiotics is a major problem in the therapeutic process. Therefore, this study was conducted to determine the antibiotic resistance and to investigate the prevalence of bla-TEM gene in K.pneumoniae isolated from urinary tract infections.
Methods: This cross-sectional descriptive study was performed on 120 samples of K.pneumonia
isolated from children with urinary tract infection referring to hospitals in Tabriz. Samples were isolated and identified using standard laboratory methods and microbiology and culture in specific environments. Antibiotic resistance patterns of bacteria isolated by Kirby-Bauer disc diffusion method were performed. In order to identify the bla-TEM gene, the combined disk test was used as a phenotypic method and PCR method was used to identify the genotypes.
Results: The highest sensitivity to the antibiotics was Tazocin(88%) and Aztreonam (87%). The combined test results showed that 26 isolates producing Extended-Spectrum beta-lactamases, of which 22 were isolates with the bla-TEM beta-lactamase gene.
Conclusions: By increasing the prevalence of resistance to antibiotics, rapid and timely diagnosis
of antibiotic-resistant strains is necessary in order to select appropriate treatments and to prevent the spread of resistance.

Keywords

Klebsiella pneumonia; Urinary Tract Infections; bla-TEM Gene

Study of Antibiotic Resistance and Prevalence of bla-TEM gene in Klebsiella pneumoniae Strains isolated from Children with UTI in Tabriz Hospitals


Authors

Abolfazl Jafari-Sales*

E-mail: [email protected]
Affiliation: Department of Microbiology, Kazeroon branch, Islamic Azad University, Kazeroon, Iran

Yashar Bagherizadeh

Affiliation: Department of Microbiology, Kazeroon branch, Islamic Azad University, Kazeroon, Iran

Parastoo Arzani-Birgani

Affiliation: Department of Microbiology, Kazeroon branch, Islamic Azad University, Kazeroon, Iran

Masume Shirali

Affiliation: Department of Microbiology, Kazeroon branch, Islamic Azad University, Kazeroon, Iran

Ahmmad Reza Shahniani

Affiliation: Department of Microbiology, Kazeroon branch, Islamic Azad University, Kazeroon, Iran

Study of Antibiotic Resistance and Prevalence of bla-TEM gene in Klebsiella pneumoniae Strains isolated from Children with UTI in Tabriz Hospitals


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Study of Antibiotic Resistance and Prevalence of bla-TEM gene in Klebsiella pneumoniae Strains isolated from Children with UTI in Tabriz Hospitals


Citation tools

Vancouver

Jafari-Sales A, Bagherizadeh Y, Arzani-Birgani P, Shirali M, Shahniani A. Study of Antibiotic Resistance and Prevalence of bla-TEM gene in Klebsiella pneumoniae Strains isolated from Children with UTI in Tabriz Hospitals. Focus on Sciences. 2106; 4(1):1-5


APA

Jafari-Sales, A., Bagherizadeh, Y., Arzani-Birgani, P., Shirali, M., & Shahniani, A. (2106). Study of Antibiotic Resistance and Prevalence of bla-TEM gene in Klebsiella pneumoniae Strains isolated from Children with UTI in Tabriz Hospitals. Focus on Sciences, 4(1), 1-5.


Chicago

Abolfazl Jafari-Sales, Yashar Bagherizadeh, Parastoo Arzani-Birgani, Masume Shirali, and Ahmmad Reza Shahniani "Study of Antibiotic Resistance and Prevalence of bla-TEM gene in Klebsiella pneumoniae Strains isolated from Children with UTI in Tabriz Hospitals". Focus on Sciences 4, no. 1 (2106).

Study of Antibiotic Resistance and Prevalence of bla-TEM gene in Klebsiella pneumoniae Strains isolated from Children with UTI in Tabriz Hospitals


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